XB-IMG-45263
Xenbase Image ID: 45263
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Fig. 3. Cres expands the signalling area of Wnt8 through extracellular interactions. (A-C) TOP-FLASH reporter analysis. The TOP-FLASH reporter was co-injected with mRNAs for Wnt8 and Cres into one blastomere (A), or separately injected into different blastomeres (B) of four-cell stage Xenopus embryos as indicated. GPI-anchored Cres was used as an immobilised form (C). Luciferase (luc) activity was assayed at the gastrula stage. Combinations of injected mRNAs are indicated (by +). Amounts of injected mRNAs (pg/embryo) were: Wnt8, 25 in A,C; Wnt8-Myc, 33 in B; cres mRNA, 0.33, 1.0, 3.3 and 10 in A and 0.10, 0.33, 1.0, 3.3 and 10 in B,C). **P<0.01, ***P<0.001 (t-test); error bars, s.e.m.; n=10 samples, except 4 (n=9) in C. (D) Expansion of Wnt8 signalling as assayed by otx2 expression. Wnt8 was expressed by injecting a DNA expression construct (pCS2+Xwnt8, 25 pg/embryo) together with nβ-gal (pCS2+nβ-gal, 100 pg/embryo; red cells). frzb or cres mRNA (1.0 pg/embryo) was injected into the blastomere diagonal to that expressing Wnt8. otx2 expression was visualised by whole-mount in situ hybridisation. The green double-headed arrows indicate the limits of the regions of otx2 inhibition. Image published in: Mii Y and Taira M (2009) Copyright © 2009. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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