XB-IMG-45320
Xenbase Image ID: 45320
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Fig. 7. Gbx2 is upstream of Pax3 and Msx1 in the NC genetic cascade. Embryos were injected as indicated and the expression of the indicated genes was analysed at stage 12. (A-F) Gbx2 MO alone (16 ng) (A,D) or co-injected with 1 ng of Pax3 mRNA (B,E) or 1 ng of Msx1 mRNA (C,F). Eighty-one to 83% of NC inhibition by Gbx2 MO (n=178) was rescued to less than 1% of inhibition by co-injection with Pax3 (n=78) or Msx1 (n=69) mRNA. (G-I) Pax3 MO alone (20 ng) (G) or co-injected with 1 ng of Gbx mRNA (H,I). Seventy-eight percent (n=90) of NC inhibition by Pax3 MO was not rescued by Gbx2 MRNA (75-79% inhibition; n=189). (J-L) Msx1 dominant-negative HD-Msx1 alone (1 ng) (J) or co-injected with 1 ng of Gbx2 mRNA (K,L). Sixty-eight percent (n=89) of NC inhibition by HD-Msx1 alone was not rescued by Gbx2 mRNA (73-77% of inhibition; n=124). Image published in: Li B et al. (2009) Copyright © 2009. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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