Xenbase Image ID: 45321

Fig. 8. Gbx2 is required for the posteriorization of neural folds. Embryo injections and expression of indicated genes were analysed at stage 12. (A,B) Snail2. (C,D) FoxD3. (E,F) Six1. Arrows, posterior end of Six1 expression. (G) RT-PCR of animal caps analysing Six1 and FoxD3 expression. (H,I) Cpl1. (J,K) En2. (L,M) Otx2. (N) Sox2. (O) Keratin. (P,Q) Summary phenotypes produced by Gbx2 MO (P) and Gbx2 mRNA (Q). Anterior part of the embryo is represented, with left side as control and right-hand side as that injected. Percentages of phenotypes are shown in Fig. S1 in the supplementary material. A minimal of 35 embryos was analysed in each experiment. AC, animal cap; E, whole embryo; Gb, 1 ng of Gbx2 mRNA; ODC, loading control; tBR, 2 ng of dominant-negative of BMP4 receptor. Image published in: Li B et al. (2009) Copyright © 2009. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue snai2.L slug, snai2-a, snai2-b, Snail2, xSlu, xslug, XSnail2 X. laevis Throughout NF stage 12 neural plate border foxd3.L fkh6, foxd3-a, foxd3-b, XFD-6, xfoxd3 X. laevis Throughout NF stage 12 neural plate border six1.L XSix1 X. laevis Throughout NF stage 12 ectoderm ptgds.S cerebrin-28, cpl-1, cpl1, l-pgds, lipocalin, lpgds, pds, pgd2, pgds, pgds2, ptgds-a, ptgds-b, XlCpl1 X. laevis Throughout NF stage 12 anterior neural ridge en2.L En-2, en2-a, en2-b, eng2, engrailed 2, engrailed homolog 2, engrailed-2, maben, xen2 X. laevis Throughout NF stage 12 presumptive midbrain-hindbrain boundary otx2.S otx-2, otx2-a, otx2-b, otxA, Xotx-2, Xotx2 X. laevis Throughout NF stage 12 neural plate sox2.L anop3, LOC108718087, mcops3, Sox-2, XLSOX-2, Xsox-2, XSox2 X. laevis Throughout NF stage 12 neural plate krt12.4.L ck81, cytok, cytokeratin, E-keratin, epi-keratin, epidermal keratin, Epidermis-specific keratin, EpiK, epiker, EpK, epK, K81, xK81, xk81a, xk81a1 X. laevis Throughout NF stage 12 ectoderm epidermis Image source: Published Larger Image Printer Friendly View

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