Xenbase Image ID: 45749

Fig. 3. Whole-mount in situ hybridization analysis of XHR1 expression during early development. (A) Dorsal view of a stage 10.5 embryo. The animal side is to the top. XHR1 expression is detected in a narrow area of the dorsal side. (B) Comparison of the expression domains of Sox-2 and XHR1. A stage 10.5 embryo was bisected sagittally into two hemispheres and was then hybridized with Sox-2 (left panel) or XHR1 (right panel). The animal side is to the top. The inset shows the higher magnification view of XHR1 expression in the sensorial layer. (C) Dorsal view of a stage 12 embryo. Anterior is to the top. The expression domain is sharpened and reduced in the midline, giving a V-shaped form. (D) Dorsal view of a stage 15 embryo. Anterior is to the top. XHR1 expression is confined to a limited area in the anterior neuroectoderm. (E) Dorsal view of a stage 17 embryo stained with XHR1 (brown) and En-2 (purple). Anterior is to the top. The expression of both XHR1 and En-2 overlaps. (F) Dorsoanterior view of a cleared stage 18 embryo. XHR1 is expressed in the prospective eye region (white arrowhead) and possible pineal gland region (white arrow) too. (G) Lateral view of a cleared stage 28 embryo. Anterior is to the left. XHR1 expression is restricted in the MHB region with slight expression in forebrain. Image published in: Shinga J et al. (2001) Copyright © 2001. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue hes7.1 HES-related 1, XHR1 Xenopus Throughout NF stage 10.5 dorsal sensorial layer of neurectoderm hes7.1 HES-related 1, XHR1 Xenopus Throughout NF stage 12   hes7.1 HES-related 1, XHR1 Xenopus Throughout NF stage 15 anterior neuroectoderm hes7.1 HES-related 1, XHR1 Xenopus Throughout NF stage 18 eye primordium hes7.1 HES-related 1, XHR1 Xenopus Throughout NF stage 28 forebrain midbrain hindbrain Image source: Published Larger Image Printer Friendly View

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