Xenbase Image ID: 45777

Fig. 3. Spatial distribution of BMP-2 and BMP-4 transcripts during embryogenesis. The spatial expression of X. tropicalis BMP-2 (A–F) and BMP-4 genes (G–L) was analysed by whole mount in situ hybridization (Harland, 1991). Digoxygenin-labelled antisense probes were derived from the translated regions of exon III, generated by PCR and subcloned into pSPT 18 (Roche). (A) morula, (B) neurula (anterior view), (C) end of neurulation, (D,E) early and late organogenesis, respectively, (F) hatching embryo, (G) midgastrula (vegetal view), (H) early neurula (posterior view), (I) end of neurulation, (J,K) early and (L) late organogenesis stages, respectively. Embryos shown in (C–F) and (I–L) are positioned with anterior to the right and posterior to the left side. All embryos have been de-pigmented in 10% H2O2/methanol and embryos shown in (I–K) are cleared in benzylbenzoate. bs, blastoporal slit; cg, cement gland; dl, dorsal lip; e, eye; h, heart; hf, heart field; hm, head mesenchyme; lpm, lateral plate mesoderm; nc, neural crest; ol, olfactory placodes; ov, otic vesicle; pg, pineal gland. Image published in: Knöchel S et al. (2001) Copyright © 2001. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue bmp4 bmp2b, bmp2b1, bmp-4, bmp4-a, bmp4-b, DVR-4, ofc11, XBMP-4, xbmp4, zyme X. tropicalis Throughout NF stage 10.5 to NF stage 12.5 mesoderm ventro-lateral marginal zone lower blastopore lip bmp2 BMP-2, bmp2-a, bmp2-b, xBMP-2, xbmp2 X. tropicalis Sometime during NF stage 18 to NF stage 20 ventral cardiac progenitor cell cardiac neural crest bmp4 bmp2b, bmp2b1, bmp-4, bmp4-a, bmp4-b, DVR-4, ofc11, XBMP-4, xbmp4, zyme X. tropicalis Throughout NF stage 21 cardiac progenitor cell head mesoderm periocular region anterior ventral blood island posterior ventral mesoderm bmp2 BMP-2, bmp2-a, bmp2-b, xBMP-2, xbmp2 X. tropicalis Throughout NF stage 22 cardiac progenitor cell cardiac neural crest ventral blood island ventral mesoderm bmp4 bmp2b, bmp2b1, bmp-4, bmp4-a, bmp4-b, DVR-4, ofc11, XBMP-4, xbmp4, zyme X. tropicalis Throughout NF stage 25 cement gland otic vesicle heart primordium cardiac mesoderm head bmp2 BMP-2, bmp2-a, bmp2-b, xBMP-2, xbmp2 X. tropicalis Throughout NF stage 27 heart pineal gland heart primordium bmp2 BMP-2, bmp2-a, bmp2-b, xBMP-2, xbmp2 X. tropicalis Throughout NF stage 28 to NF stage 29 and 30 cardiac mesoderm heart primordium olfactory placode lateral plate mesoderm anterior dorsal lateral plate region primary heart field secondary heart field bmp2 BMP-2, bmp2-a, bmp2-b, xBMP-2, xbmp2 X. tropicalis Throughout NF stage 8 ectoderm animal cap Image source: Published Larger Image Printer Friendly View

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