Xenbase Image ID: 45863

Fig. 5. Expression of opl in the late blastula ectoderm is uniform and UV-insensitive, while dorsal localization of opl and fkh5 in early gastrula is prevented by UV. Whole-mount in situ hybridization is shown for opl in normal embryos or embryos irradiated with UV light during the first cell cycle. In (c,e,g,i) dorsal is towards the top. All views are of the animal hemisphere for opl in situ hybridizations, and of the vegetal pole for fkh5 in situ hybridizations. (a) Expression of opl in control stage 9.5 (late blastula) embryos. (b) Expression of opl at stage 9.5 in embryos treated with UV light during the first cell cycle. (c) Expression of opl in control stage 10.5 (early gastrula) embryos. (d) Expression of opl at stage 10.5 in UV-treated embryos. (e) Expression of fkh5 in control stage 10.5 embryos. (f) Expression of fkh5 at stage 10.5 in UV-treated embryos. (g) Expression of opl in control stage 11.5 (mid-gastrula) embryos. (h) Expression of opl at stage 11.5 in UV-treated embryos. (i) Expression of fkh5 in control stage 11.5 embryos. (j) Expression of fkh5 at stage 11.5 in UV-treated embryos. Expression of opl at stage 9.5 is unaffected by UV treatment, but dorsal localization of opl and fkh5 expression at stage 10.5 and 11.5 is prevented by UV. Embryos harvested at stage 30 equivalent showed that the UV treatment was effective, with all embryos having a dorso-anterior index of 0 or 1 (data not shown). Embryos hybridized to an opl sense probe, as a control for non-specific staining, showed no staining (data not shown). Image published in: Gamse JT and Sive H (2001) Copyright © 2001. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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