Xenbase Image ID: 45946

Fig. 3. Loss of Xfoxi1a/b function results in an expansion of the neural plate and reduction of non-neural ectodermal tissues. (A) The Xfoxi1a/b-MO binding sites are shown with the underlines. The red box indicates the start codon. Identical nucleotides are marked with asterisks. (B) Flag-tagged Xfoxi1a mRNA (50 pg/cell) was injected with Xfoxi1a-MO (2.5 ng/cell), Xfoxi1b-MO (2.5 ng/cell) or the control five-base mismatched Xfoxi1a MO into animal cells of eight-cell embryos. (C) Flag-tagged Xfoxi1b mRNA (50 pg/cell) was injected with Xfoxi1b-MO (2.5 ng/cell), Xfoxi1a-MO (2.5 ng/cell) or control five-base mismatched Xfoxi1b MO into animal cells of eight-cell embryos. (D) Flag-tagged δ5′UTR-Xfoxi1a mRNA (50 pg/cell) was injected with Xfoxi1a-MO (2.5 ng/cell) or Xfoxi1b-MO (2.5 ng/cell) into animal cells of eight-cell embryos. Animal caps were excised at stage 9 and cultured until stage 11. Xfoxi1a-flag (B,D) or Xfoxi1b-flag (C) proteins were detected by western blot analysis using an anti-flag antibody. Hsp70 was used as the loading control.δ 5′UTR means that the synthetic mRNA contains only the coding sequence and not the target sequence of Xfoxi1a/b-MO. (E-U) Xfoxi1a-MO (12.5 ng/cell; i1aMO; E-M), five-base mismatched control MO of Xfoxi1a (12.5 ng/cell; 5mis; N-P), Xfoxi1a-MO (12.5 ng/cell) ± Xfoxi1a mRNA (25 pg/cell; Q,R) or Xfoxi1b-MO (12.5 ng/cell; i1bMO; S-U) was injected into two unilateral blastomeres of eight-cell embryos. Embryos were harvested at stage 14-15 (E-J,L-U) or stage 24 (K) and analyzed by whole-mount in situ hybridization with the probes indicated in each panel. (E-H,J,L-O,Q-T) Dorsal view (anterior towards the top); (I,K,P,U) anterior view (dorsal towards the top). Injected sides are marked with arrowheads. Dashes indicate the midline. NP, nasal placode. Double-headed arrows in G,Q show the expansion of Dlx3– and Six2+ regions, respectively. Image published in: Matsuo-Takasaki M et al. (2005) Copyright © 2005. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue sox2.L anop3, LOC108718087, mcops3, Sox-2, XLSOX-2, Xsox-2, XSox2 X. laevis Sometime during NF stage 14 to NF stage 15 neural plate krt12.5.S krt12, krt12.1, larval keratin, xk81b2 X. laevis Sometime during NF stage 14 to NF stage 15 epidermis dlx5.L dll3, X-dll3 X. laevis Sometime during NF stage 14 to NF stage 15 epidermis foxd3.L fkh6, foxd3-a, foxd3-b, XFD-6, xfoxd3 X. laevis Sometime during NF stage 14 to NF stage 15 neural crest ag1.L ag, ag1-a, ag1-b, XAG, xag-1, xag1, Xag2 X. laevis Sometime during NF stage 14 to NF stage 15 cement gland primordium six1.L XSix1 X. laevis Sometime during NF stage 14 to NF stage 15 preplacodal ectoderm pre-chordal neural plate six1.L XSix1 X. laevis Throughout NF stage 24 olfactory placode chrd.S chd, chordin, chrd.1, LOC108716939, X-chordin X. laevis Sometime during NF stage 14 to NF stage 15 axial mesoderm myod1.S mf25, MyoD, myod1-a, myod1-b, myogenic factor 3, XMyoD, XtmyoD X. laevis Sometime during NF stage 14 to NF stage 15 paraxial mesoderm Image source: Published Larger Image Printer Friendly View

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