Xenbase Image ID: 46019

Fig. 8. Expression of Nieuwkoop, BCNE or Spemann organizer genes is not restored inβ -Catenin/Bmp4/Bmp7-depleted embryos. (A-D) Expression of Xnr6 in the vegetal dorsal side at blastula stage 9 in the Nieuwkoop center requires β-Catenin, is unaffected by knockdown of Bmp4/Bmp7, and is not re-expressed in β-Catenin/Bmp4/Bmp7 triple knockdowns. (E-L) Expression of the BCNE center genes Pintallavis/Hnf3β and Chordin at blastula stage 9 requires β-Catenin, is not affected by the depletion of Bmp4/Bmp7 and fails to be rescued in β-Catenin/Bmp4/Bmp7 triple knockdowns. (M-P) Chordin expression in the Spemann organizer at gastrula stage 10 is eliminated by β-Catenin depletion but is unchanged in Bmp4/Bmp7-depleted embryos. Note that embryos shown in panels D,H,L and P do not express any of the dorsal genes tested, yet still give rise to hyperdorsalized embryos with large head structures. (Q) RT-PCR analysis at stage 9. Dorsal markers are not expressed in embryos lacking β-Catenin orβ -Catenin/Bmp4/Bmp7. (R-V) A simplified model highlighting the interactions taking place at blastula stage between BMP signals and the dorsal BCNE and Nieuwkoop centers. Image published in: Reversade B et al. (2005) Copyright © 2005. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue nodal6.L nodal6-a, nodal6-b, nr6, xnr6, xnr6b X. laevis Throughout NF stage 9 vegetal dorsal foxa4.L fkh1, foxa4-a, foxa4-b, FoxA4a, pintallavis, XFD-1, xfkh1, XFKHI, xkfh-1 X. laevis Throughout NF stage 9 endoderm mesoderm dorsal dorsal marginal zone chrd.S chd, chordin, chrd.1, LOC108716939, X-chordin X. laevis Throughout NF stage 9   chrd.S chd, chordin, chrd.1, LOC108716939, X-chordin X. laevis Throughout NF stage 10 to NF stage 12.5 marginal zone Image source: Published Larger Image Printer Friendly View

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