Xenbase Image ID: 46138

Fig. 2. XRALDH2 is differentially expressed during Xenopus development. (A) XRALDH2 mRNA levels during Xenopus embryogenesis and in adult tissues revealed by RT-PCR. RT-PCR analysis with RNA preparations from staged embryos (embryonic stages indicated according to Nieuwkoop and Faber (1967)) and from adult tissues. Histone H4 was used as an RNA loading control. (B) Whole-mount in situ hybridization analysis using XRALDH2 antisense RNA. (1–3) Posterior view (top, dorsal; bottom, ventral); (4), dorsal view (top, anterior; bottom, posterior); (5,6), dorsal and ventral views, respectively (left, anterior; right, posterior); (7–10), anterior view; (11), lateral view; (2a), sagittal section of a stage 11 embryo; (4a,11a,11b), transverse sections (top, dorsal; bottom, ventral); (10a), the section plane is indicated by the red rectangle in panel 10. (C) Double staining whole-mount in situ hybridization of XRALDH2 (red) and XCYP26 (blue). (1–3), posterior view (top, dorsal; bottom, ventral); (4), dorsal view (top, posterior; bottom, anterior); (5), anterior view; (6), lateral view; (2a), sagittal section; (6a), higher magnification of the head region of 6; (6b, 6c), frontal section (top, dorsal; bottom, ventral); (6d), horizontal section (top, anterior; bottom, posterior). Red dashed lines and rectangles in (B) and (C) indicate the locations for vibratome sections. Nieuwkoop–Faber stages of embryogenesis are indicated in the lower right corner of each picture ( Nieuwkoop and Faber, 1967). (B,C) ar, Archenteron; cg, cement gland; dc, diencephalon; dz, deep zone of involuting marginal zone; ec, ectoderm; el, epithelial layer of involuting marginal zone; ele, epithelial layer of ectoderm; ls, lens; m, mesoderm; mc, mesencephalon; md, developing mesonephric duct; mg, midgut; m/h, midbrain/hindbrain boundary expression of XCYP26; no, notochord; oe, oral epithelia; op, olfactory placode; pc, prosencephalon; pe, pigment epithelium of retina; r3, rhombomere 3 domain of XCYP26; rc, rhombencephalon; re, retina; sc, spinal cord; sh, stomodeal–hypophyseal anlage; sl, sensorial layer of ectoderm; zii, zone of internal involution. Image published in: Chen Y et al. (2001) Copyright © 2001. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue aldh1a2.L raldh-2, raldh2, XRaldh2 X. laevis Sometime during NF stage 10 to NF stage 10.5 upper blastopore lip marginal zone involuting marginal zone aldh1a2.L raldh-2, raldh2, XRaldh2 X. laevis Sometime during NF stage 11 to NF stage 11.5 involuted dorsal mesoderm involuting marginal zone aldh1a2.L raldh-2, raldh2, XRaldh2 X. laevis Throughout NF stage 14 mesoderm lateral plate mesoderm intermediate mesoderm presomitic mesoderm aldh1a2.L raldh-2, raldh2, XRaldh2 X. laevis Throughout NF stage 15 mesoderm presomitic mesoderm lateral plate mesoderm optic field olfactory region aldh1a2.L raldh-2, raldh2, XRaldh2 X. laevis Throughout NF stage 16 optic vesicle stomodeal-hypophyseal primordium lateral plate mesoderm intermediate mesoderm aldh1a2.L raldh-2, raldh2, XRaldh2 X. laevis Throughout NF stage 25 retinal pigmented epithelium retina stomodeal-hypophyseal primordium olfactory placode roof plate otic placode aldh1a2.L raldh-2, raldh2, XRaldh2 X. laevis Throughout NF stage 35 and 36 ciliary marginal zone retinal pigmented epithelium sensorial layer pronephric kidney lateral plate mesoderm pronephric duct otic vesicle aldh1a2.L raldh-2, raldh2, XRaldh2 X. laevis Throughout NF stage 18 optic field lateral plate mesoderm intermediate mesoderm aldh1a2.L raldh-2, raldh2, XRaldh2 X. laevis Throughout NF stage 21 optic field stomodeal-hypophyseal primordium lateral plate mesoderm intermediate mesoderm aldh1a2.L raldh-2, raldh2, XRaldh2 X. laevis Throughout NF stage 12 involuting marginal zone involuted dorsal mesoderm aldh1a2.L raldh-2, raldh2, XRaldh2 X. laevis Throughout NF stage 13 lateral plate mesoderm intermediate mesoderm aldh1a2.L raldh-2, raldh2, XRaldh2 X. laevis Throughout NF stage 20 intermediate mesoderm lateral plate mesoderm stomodeal-hypophyseal primordium optic field aldh1a2.L raldh-2, raldh2, XRaldh2 X. laevis Throughout NF stage 33 and 34 pronephric duct pronephric kidney otic vesicle ciliary marginal zone lateral plate mesoderm cyp26a1.L cyp26, RACE, retinoic acid converting enzyme, xCYP26, XCYP26A1 X. laevis Throughout NF stage 33 and 34 somite branchial arch optic field tail bud olfactory region stomodeal-hypophyseal primordium Image source: Published Larger Image Printer Friendly View

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