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Fig. 1. Wee1-depletion using antisense morpholino oligonucleotides (MO). (A) MO-Wee1 was injected into both cells of a two-cell embryo. Uninjected and MO-Wee1-injected embryos were collected at various stages and lysates examined by immunoblot analysis using anti-Wee1 antibodies (* indicates a nonspecific band). (B) Two-cell embryos were injected with MO-Control, MO-Wee1 or co-injected with MO-Wee1 and WT Wee1 RNA (MO-Wee1+RNA). Embryos were collected at stage 10 and lysates examined as in A. (C) Embryos injected as in B were collected at stage 8, 10 or 12. Lysates were examined by immunoblot analysis using anti-phospho-Cdc2 or anti-Cdc2 antibodies. (D) The mitotic nuclei of injected stage 11 embryos were visualized by whole-mount immunostaining using phospho-histone H3 (αPH3). Mitotic index (n=10-12 embryos): MO-control, 8.9% (4739 nuclei); uninjected, 9.9% (1221 nuclei); MO-Wee1, 24.5% (4734 nuclei); MO-Wee1+RNA, 11.4% (2736 nuclei). Image published in: Murakami MS et al. (2004) Copyright © 2004. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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