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Fig. 7. Cdc25C overexpression disrupts gastrulation. (A,B) The two dorsal blastomeres of four-cell embryos were injected with β-gal RNA (4 ng) or co-injected with β-gal (100 pg) and His-Cdc25C (3 ng) and embryos were scored for gastrulation defects at stage 11.5-12. Number of embryos examined: Cdc25C (n=141), β-Gal (n=162). (A, lower panels) Stage 10.5-11 embryo lysates were examined by immunoblot analysis using anti-His-epitope, Cdc2 and phospho-Cdc2 antibodies. (C) Following injection as in A, mitotic nuclei of stage 11 embryos were visualized. Mitotic index (n=10-12 embryos): β-gal, 8.3% (6928 nuclei), Cdc25C, 28.1% (5550 nuclei). (D,E) Embryos were injected with 4 ng of Cdc25C RNA and either 0, 0.2 or 0.5 ng of Wee1 RNA. Number of embryos examined: Cdc25C + 0 ng Wee1 (n=74), Cdc25C + 0.2 ng Wee1 (n=100) and Cdc25C + 0.5 ng Wee1 (n=86). Note that increased expression of Wee1 counteracts the defects induced by Cdc25C overexpression. Image published in: Murakami MS et al. (2004) Copyright © 2004. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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