Xenbase Image ID: 46888

Figure 3. Movement of XHex-expressing cells from a central domain to the dorsal side of the embryo. Cells in a central region of the blastocoel floor marked with Dil at stage 9 moved to the dorsal side of the embryo by stage 10.5, and came to underlie anterior ectoderm adjacent to the ventrally displaced blastocoel by stage 11.5. (a) An embryo fixed shortly after labelling a central population of cells at the floor of the blastocoel. (b) An embryo (stage 10.5) analysed after formation of a crescent-shaped dorsal blastopore lip (arrow). Embryos at this stage were recovering from manipulation and were somewhat distorted. Nevertheless, labelled cells were always positioned on the dorsal side of the embryo above the blastopore lip. (c–e) Embryos fixed at later gastrula stages (stage 11–11.5), after recovery from surgery. Labelled cells were positioned in anterior endodermal tissue, immediately adjacent to the ventrally displaced blastocoel (BLC). (f) Drawing of the specimen in (e) to show the position of labelled cells with respect to the germ layers of the embryo; 55 embryos were analysed in this experiment. (g,h) Two examples of embryos that were ventralised by treatment with ultraviolet (UV) radiation. Central blastomeres were labelled and the embryos analysed at early gastrula stages. Cells did not move from their original central location in UV-treated embryos. Image published in: Jones CM et al. (1999) Copyright © 1999. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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