XB-IMG-46922
Xenbase Image ID: 46922
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Figure 1. XDbf4 Associates with Frodo and Is Expressed throughout Xenopus Development(A) Diagram of XDbf4 and XDbf4-δMC with amino acid numbers indicated. Domains N, M, and C conserved between XDbf4 and yeast Dbf4 are shown.(B) XDbf4 and XDbf4-δMC coimmunoprecipitate with Frodo. Lysates of embryos injected with Myc-tagged XDbf4 or XDbf4-δMC (δMC) and HA-tagged FrdB mRNA were precipitated with anti-HA antibody as indicated. Top panel, Myc-XDbf4 and Myc-δMC coprecipitated with FrdB was detected with anti-Myc antibodies; middle panel, precipitated HA-FrdB detected with anti-HA antibodies; bottom panel, Myc-XDbf4 and Myc-δMC detected with anti-Myc antibodies in whole lysates. C, uninjected control embryos.(C–N) In situ hybridization analysis of XDbf4 expression. (C–G, I, and J) XDbf4 antisense probe. (H and K) Chordin antisense probe as a specificity control. (C) Stage 9, animal pole view. (D) Stage 9, lateral view. (E) Stage 10.5, gastrula, dorsal view. (F) Stage 20, late neurula, dorsal view. (G) Stage 22, lateral view. (H) Stage 10, vegetal view. (E and H) Arrowheads point to dorsal lip. (I and K) Stage 26. (J) Stage 35/36. (F, G, and I–K) Anterior is to the left. (L, M, and N) Sections from the regions indicated in (I) and (K); dorsal is up. ha, heart anlage; e, eye vesicle; en, endodermal yolk mass; sa, stomodeal anlage; ms, mesencephalon; fg, foregut; nc, notochord. Bar in L, 200 μm, also corresponds to M and N.(O) Developmental expression of XDbf4 protein. Western analysis of embryo lysates with anti-XDbf4 antibodies was carried out at indicated stages. A vertical bar indicates multiple phosphorylation states of XDbf4; asterisk, a nonspecific protein band reflects loading; M.W., molecular weight markers. Image published in: Brott BK and Sokol SY (2005) Copyright © 2005. Image reproduced with permission of the Publisher, Elsevier B. V.
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