Xenbase Image ID: 46931

Figure 3. Regulation of Xenopus R-spondins by Wnt Signaling(A) Comparison of XRspo2, XWnt8, and XWnt3A expression pattern in early neurula embryos by whole-mount in situ hybridization. Dorsal view, anterior up.(B) Top: Diagram of experiment. 4-cell stage embryos were injected with 50 pg pCS-ppl (preprolactin), pCS-XWnt8, or pCS-β-catenin into each blastomere, and DMZs were dissected, cultured until stage 11 equivalent, and analyzed by RT-PCR. Bottom: RT-PCR analysis of the indicated genes. −RT, minus reverse transcription control.(C–E) 4-cell stage embryos were injected with 50 pg pCS-Wnt8, pCS-Wnt3A, or pCS-ppl into one blastomere and fixed at stage 11 for in situ hybridization with XRspo2. Image published in: Kazanskaya O et al. (2004) Copyright © 2004. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue rspo2.L CRISTIN2, R-spondin 2 X. laevis Throughout NF stage 15 neural fold anterior neural fold Rohon-Beard neuron anterior placodal area ectoderm neuroectoderm intermediate mesoderm wnt8a.L wnt-8, wnt8, Xwnt-8, Xwnt8 X. laevis Throughout NF stage 15 neural fold anterior neural fold neuroectoderm wnt3a.S wnt-3a, Xwnt-3a, xwnt3a X. laevis Sometime during NF stage 13 to NF stage 14 neural fold anterior neural fold posterior Image source: Published Larger Image Printer Friendly View

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