Xenbase Image ID: 46950

Figure 2. Analysis of Developmental Gene Expression in Nonregenerating and Regenerating Tail TissuesIn situ hybridization of genes involved in tail development.(A, E, I, M, Q, and U) Stage 30 embryos.(B, F, J, N, R, and V) Nonregenerating tail stumps, 50% amputated at stage 47 and fixed 3 days afterward.(C, G, K, O, S, and W) Stage 49 tail stumps, 50% amputated and fixed immediately.(D, H, L, P, T, and X) Regenerating tail stumps, 50% amputated at stage 49 and fixed 3 days later. Amputation level is shown by the black line.(A–D) Xbra is expressed in the developing tail bud (yellow arrow) and reexpressed in the tip of regenerating tails (white arrow).(E–H) X-delta-1 is expressed in the posterior wall of the tail bud (yellow arrow) and fin and is reexpressed in the fin and bud region (white arrow) of regenerating tails.(I–L) lfng is expressed in the dorsal tail bud (yellow arrow) and is expressed weakly at the cut surface of the spinal cord in nonregenerating tail stumps and more strongly in dorsal components of the regenerating tail (white arrows).(M–P) X-Notch-1 is expressed in somites, fin margin, and throughout the developing tail bud (yellow arrow) and is reexpressed in the blastema (white arrow), presomitic mesoderm, and fin margin.(Q–T) Msx2 is expressed in the tail bud leading edge (yellow arrow) and reexpressed throughout the blastema (white arrow) and fin margin.(U–X) Msx1 is expressed in the dorsal tail bud (yellow arrow) and reexpressed dorsally in the regenerating blastema (white arrow). All are lateral views, oriented with anterior to the left. Image published in: Beck CW et al. (2003) Copyright © 2003. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue tbxt.S bra, brachyury, ntl, t, t-a, t-b, X-bra, Xbra, Xbrachyury X. laevis Throughout NF stage 29 and 30 regenerating tail tail bud tail tip dll1.L delta-1, Delta-1, delta1, x-delta, X-delta-1, Xdelta-1, Xdelta1, XDelta1 X. laevis Throughout NF stage 29 and 30 notochord regenerating tail eye brain forebrain midbrain hindbrain tail bud cement gland pharyngeal arch ventral fin margin lfng.L lunatic fringe X. laevis Throughout NF stage 29 and 30 forebrain midbrain regenerating tail brain hindbrain eye pronephric duct pronephric kidney lateral plate mesoderm dorsal tail bud anterior ventral blood island spinal cord notch1.S notch, notch-1, x-notch-1, xnotch, xnotch1, xotch X. laevis Throughout NF stage 29 and 30 mesoderm presomitic mesoderm somite regenerating tail otic vesicle eye pharyngeal arch heart dorsal fin margin ventral fin margin tail tip pronephric kidney pronephric duct brain forebrain midbrain hindbrain msx2.L Hhox 7.1 X. laevis Throughout NF stage 29 and 30 tail bud fin regenerating tail dorsal fin margin ventral fin margin ventral tail fin ventral blood island msx1.L hox-7, hox7, msx-1, msx1-a, msx1-b, Xhox-7.1, Xmsx-1, xmsx1 X. laevis Throughout NF stage 29 and 30 fin blastema regenerating tail brain forebrain midbrain hindbrain pharyngeal arch eye otic vesicle anterior neural tube dorsal tail bud dorsal fin margin ventral fin margin head region Image source: Published Larger Image Printer Friendly View

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