Xenbase Image ID: 46958

Figure 4. c-Myc Depletion Does Not Perturb A/P Patterning(A) Expression of sox10 in migratory cranial neural crest cells ([i], arrows) is absent on the c-Myc-depleted side of the embryo (ii).(B) (i) Expression of sox10 in the ear (arrows) and CNS is unperturbed on the c-Myc-depleted side of embryos (red arrowhead) and remains colinear with expression on the control side (white arrowhead). (ii) Expression of krox20 in migratory neural crest cells is absent on the injected side of the embryo (black arrow), while expression in the CNS is unperturbed. CNS expression remains colinear with the uninjected side.(C) In situ hybridization with a muscle actin probe demonstrates that c-Myc MOs targeted to the mesoderm (ii) do not perturb somite development.(D) RT-PCR analysis in ectodermal explants of embryos injected with neural crest-inducing doses of Wnt and chordin. c-Myc MOs inhibit the expression of the neural crest markers slug, twist, and snail. L32 expression serves as a loading control. Image published in: Bellmeyer A et al. (2003) Copyright © 2003. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue myc c-myc, cmyc, myc-a, myc-b, X-cmyc, Xcmyc Xenopus Throughout NF stage 13 to NF stage 21 cranial neural crest sox10 Xenopus Throughout NF stage 13 to NF stage 21 auditory apparatus central nervous system Image source: Published Larger Image Printer Friendly View

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