Xenbase Image ID: 46983

Figure 3. IGF Signaling and the Head/Trunk Boundary(A) Uninjected 3 day tadpole in lateral view.(B) Embryo injected with 500 pg of DN-IGFR mRNA per animal blastomere at the 4- to 8-cell stage showing reduction of cement gland and eye structures (92%, n = 48).(C–H) The domains of expression of Otx-2 (head), XAG-1 (cement gland), and Rx2a (eye) markers are reduced by DN-IGFR mRNA.(I–N) Dorsal views of neurula stage embryos injected into one blastomere at the 4-cell stage with nuclear lacZ mRNA (note red nuclei). In situ hybridization was performed at neurula stage with probes for Six-3 (I–K) or a combination of Otx-2 and the trunk marker HoxD1 (L–N). IGF-2 (400 pg) shifts the head/trunk border caudally (n = 25) and DN-IGFR mRNA (500 pg) displaces it anteriorly (n = 15). IGF-2 and DN-IGFR mRNA injections do not significantly change the number of nuclear LacZ-labeled nuclei Image published in: Pera EM et al. (2001) Copyright © 2001. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue otx2.S otx-2, otx2-a, otx2-b, otxA, Xotx-2, Xotx2 X. laevis Throughout NF stage 17 eye primordium pre-chordal neural plate anterior neural fold ag1 ag, ag1-a, ag1-b, XAG, xag-1, xag1, Xag2 Xenopus Throughout NF stage 17 cement gland primordium rax.S rax-a, rax-b, rax1, rx, rx-1, rx1, rx1a, Rx2A, Xrax, Xrx1, Xrx1A X. laevis Throughout NF stage 17 eye eye primordium optic vesicle six3.L hpe2, six3-a, six3-b, XSix3, xsix3.1, xsix3.2 X. laevis Throughout NF stage 17 eye primordium pre-chordal neural plate anterior neural fold hoxd1.S MGC131028, Xhox.lab, xhox.lab1, xhoxd1, xlab, Xlab-1 X. laevis Throughout NF stage 17 trunk dorsal Image source: Published Larger Image Printer Friendly View

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