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FIG. 3. The Xenopus tinman homologues are expressed in distinctive patterns in the different germ layers. Embryos were stained by whole-mount in situ hybridisation for XNkx2-5 (A), XNkx2-3
G, H), and XNkx2-10 (I) expression. Transverse sections are shown in A and I and a parasaggital section in H. Expression is shown
in dark blue and nuclei are counter-stained in red with Fuelgen.
Embryos were at early tail bud (stage 26, A), heart trough (stage 27/28, B, G), linear heart tube (stage 32, C, H, I), or chamber formation stages (stage 40, D). TO, outflow tract; V, ventricle; A,
atrium; SV, sinus venosus; PE, pharyngeal endoderm; M, myocardium; DM, dorsal mesocardium; CG, cement gland; Me, mesoderm.