XB-IMG-47180
Xenbase Image ID: 47180
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Figure 1. Xenopus E-Syt2 Is Required for Induction of the Early Mesoderm Gene Xbra and for FGF Signaling(A) Predicted structural domains of E-Syt2 orthologs, “x” Xenopus, “h” human, “Dm” Drosophila melanogaster, “Ce” Caenorhabditis elegans.(B) Zygotic E-Syt2 expression depends on FGF signaling. FGF signaling was inhibited by the expression of the dominant-negative FGF receptor “XFD” (100, 200, and 400 pg) in ventral marginal zone (VMZ) explants.(C) In vivo Xbra expression is suppressed by E-Syt2 depletion. Dorsal blastomeres of four cell embryos were each injected equatorially with 0.75 pmol of Mo-C and increasing amounts of HA-E-Syt2 mRNA and Xbra expression at stage 10.5 revealed by whole-mount in situ hybridization.(D) E-Syt2 depletion suppresses ectopic induction of Xbra by activated FGFR1. Embryos were injected with 0.9 or 1.8 pmol Mo-B or Ctrl Mo and with 2.5 pg constitutively active FGFR1 (CA-FGFR1) or 5 pg VRas.(E) Activated FGFR1 (1.5 pg/cell, four cell embryos) induction of ectopic ERK activation in ACs was also suppressed by depletion of E-Syt2 (3 pmol MoC/cell).(F) Depletion of E-Syt2 also suppresses FGF8-dependent differentiation. Embryos were unilaterally coinjected with FGF8 (2.5 pg), β-galactosidase (325 pg) mRNAs, and E-Syt2 Mo-B or Ctrl Mo (2 pmol) and subjected to whole-mount in situ to reveal N-tubulin expression (blue). Purple staining (β-galactosidase activity assay) indicates injected side of embryo.See also Figure S1. Image published in: Jean S et al. (2010) Copyright © 2010. Image reproduced with permission of the Publisher, Elsevier B. V.
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