Xenbase Image ID: 47749

Figure 2. CPEB and Maskin Localization during Early Embryogenesis(A) Immunostaining for CPEB and maskin in ovulated eggs, 4 cell, and 16 cell albino embryos. The animal pole is the site of intense staining.(B) Western blot analysis of CPEB, maskin and tubulin in the animal (A) and vegetal (V) halves of stage 6 oocytes, progesterone-matured oocytes, ovulated eggs, and blastula stage embryos. Each lane was loaded with protein from one-half oocyte or embryo equivalent.(C) Embryos that were stained with CPEB and maskin antibodies, or with nonimmune serum, were treated with a benzyl-benzoate:benzyl alcohol (1:2) solution to clear the yolk. CPEB and maskin are associated with structures that resemble mitotic spindles.(D) Embryos were simultaneously immunostained for α-tubulin and either maskin, CPEB, or ElrA. Image published in: Groisman I et al. (2000) Copyright © 2000. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue cpeb1.L CPEB, Cpeb-1, cpeb1-a, cpeb1-b X. laevis Sometime during unfertilized egg stage to NF stage 5 (16-cell) animal hemisphere tacc3.S eric1, maskin, xmaskin X. laevis Sometime during unfertilized egg stage to NF stage 5 (16-cell) animal hemisphere Image source: Published Larger Image Printer Friendly View

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