Xenbase Image ID: 47754

Figure 7. Effect of CPEB Antibody, CPEB δ4, and Cordycepin Injection on the Localization and Translation of Cyclin B1 mRNA(A) Embryos injected with CPEB δ4, CPEB antibody, or cordycepin were collected following first cleavage (of the controls) and western blotted for cyclin B1 and tubulin (top). Each lane was loaded with protein from one embryo. These western blots were scanned and the relative amount of cyclin B1 at each point was quantified and plotted (bottom).(B) Fertilized eggs, some of which were injected with CPEB δ4 or CPEB antibody, were processed for cyclin B1 mRNA whole-mount in situ hybridization at the same time of embryogenesis.(C) Control and CPEB δ4 injected embryos were analyzed for α-tubulin and cyclin B1 protein by whole mount immunohistochemistry and confocal microscopy. Image published in: Groisman I et al. (2000) Copyright © 2000. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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