Xenbase Image ID: 47812

Figure 5. Effects of overexpressing ephrin-B2 on neural crest migration. RNA encoding ephrin-B2 was injected into Xenopus embryos at the two-cell stage, which were later fixed and in situ hybridisation carried out to detect second and third arch neural crest markers. (a,b) Embryos fixed at stage 20 and analysed with a Krox-20 probe. (c,d) Embryos fixed at stage 24 and analysed with a Krox-20 probe. (e,f) Embryos fixed at stage 20 and analysed with Krox-20 plus EphA2 probes. (g,h) Embryos fixed at stage 24 and analysed with an EphA2 probe. Ectopic Krox-20-expressing cells (black arrows) are detected caudal and/or rostral to the third arch neural crest stream (n3), which is often abnormal in shape. In contrast, the second arch crest (n2) marked by EphA2 expression appears normal. In some embryos (e), there was a gap between the third and second arch neural crest which could reflect a narrower stream of third arch crest cells. Image published in: Smith A et al. (1997) Copyright © 1997. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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