Xenbase Image ID: 47813

Figure 6. Effects of targeted expression in neural crest. RNA was injected into the A1 or A2 blastomeres of 32-cell embryos in order to target expression of ephrin-B2 or truncated receptors to ectodermal derivatives, including branchial neural crest. Krox-20 expression was then detected by in situ hybridisation (blue signal). In some experiments, fluorescein-dextran was co-injected as a lineage tracer and detected using alkaline-phosphatase-conjugated, anti-fluorescein antibody and Fast Red substrate (red signal in (e–h)). (a–f) Embryos injected with RNA encoding truncated EphB1 plus EphA4 shown in lateral views (a–e) and in a coronal section (f) through the embryo shown in (e). The latter embryo has some non-specific staining that is often seen using Fast Red substrate. (g,h) Embryo injected with RNA encoding ephrin-B2 shown in a lateral view (g) and coronal section (h). Ectopic Krox-20-expressing cells are indicated by black arrows, and the lineage tracer in branchial neural crest by white arrows. Image published in: Smith A et al. (1997) Copyright © 1997. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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