Xenbase Image ID: 47825
Fig. 2. Expression of Cyp26a and b during organogenesis. In situ hybridisation of Cyp26a and b, dark blue stain indicates expression. Panels A–F are Cyp26a and G–L are Cyp26b. Panels A–C and G–I are whole mount whole mount lateral views with head facing left and dorsal uppermost. (A) Expression of Cyp26a at stage 28. (B) Expression of Cyp26a at stage 30. (C) Expression of Cyp26a at stage 34. (B’) Close-up view of intersomitic staining in embryo shown in panel B. (D–F) 12 μm sagittal sections through previously stained stage 34 embryo showing Cyp26a expression in dark blue and with eosin (pink) used as a counterstain. Heads are to the left and dorsal uppermost. (D) Section through eye. (E) Deeper section (more towards midline) through head area. (F) Section through trunk somites. (G) Cyp26b expression in stage 28 embryo. (H) Cyp26b expression in stage 30 embryo. (I) Cyp26b expression in stage 34 embryo. Red arrows indicate the level of sections used in panels K and L. (H’) Closer detail of the rhombomere staining in panel H. (J) Sagittal section through stage 34 embryo showing expression in numbered rhombomeres of the hindbrain. Anterior is to the left and dorsal uppermost (J). (K) Transverse section at the level of the otic vesicles showing hindbrain staining localised towards the lumenal side. (L) Transverse section through ventral embryo at the level of the heart showing staining in ventral aortae. In K and L, dorsal is uppermost. All scale bars are100 μm. Abbreviations: ba, branchial arches; cf, choroid fissure; e, endoderm; he, heart; isv, intersomitic vessels; le, lens; lpm, lateral plate mesoderm; ov, otic vesicle; r, rhombomere; re, retina; s, somite; tb, tailbud; va, ventral aortae.
Image published in: Lynch J et al. (2011)
Copyright © 2011. Image reproduced with permission of the Publisher, Elsevier B. V.
Image source: Published
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