Xenbase Image ID: 47933

Fig. 1. Characterization of Xfurry. (A) Temporal expression of Xfurry detected by RT-PCR. Numbers indicate developmental stages. U, unfertilized eggs. (B) Regional RT-PCR of Xfurry at stage 10. (C–F) Whole-mount in situ hybridization. (C) Arrowhead indicates the dorsal lip (stage 10). (D) Arrowhead indicates the chordamesodermal region (stage 12). (E and F) Strong expression in the notochord was maintained through stage 25 (E) and stage 33 (F). (G) Control embryo (stage 33). (H) Xfurry (2 ng/blastomere) injected into ventral blastomeres of the four-cell embryo induced a secondary axis without anterior structures (arrowhead) in 63% of embryos (n = 60). (I) Expression of the chordamesodermal genes was induced in animal caps (stage 10) that were dissected from embryos injected with Xfurry (2 ng/blastomere). Gsc, goosecoid; Pint, pintallavis; Chd, chordin; ODC, Ornithine decarboxylase. (J) Control embryo (stage 26). (K) Injection of Xfurry-MO (5 ng/blastomere) into dorsal blastomeres of four-cell embryos shortened the dorsal axis in all embryos (n = 58). (L) Expression of chordamesodermal marker genes was reduced in Xfurry-MO–injected embryos, but transcripts of Xfurry were increased by Xfurry-MO (stage 10). Image published in: Goto T et al. (2010) Copyright © 2010. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue fry.L furry, Xfurry X. laevis Throughout NF stage 10 upper blastopore lip fry.L furry, Xfurry X. laevis Throughout NF stage 12 axial mesoderm fry.L furry, Xfurry X. laevis Throughout NF stage 25 notochord fry.L furry, Xfurry X. laevis Throughout NF stage 33 and 34 notochord head region pharyngeal arch branchial arch cement gland Image source: Published Larger Image Printer Friendly View

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