Xenbase Image ID: 48065

Fig. 6. Cyclopamine treatment blocks Hh signalling in Xenopus. Whole-mount in situ hybridisation on embryos incubated in cyclopamine solution from stage 20 onwards (B,D,F,H,J,L) or control embryos (A,C,E,G,I,K). The probes are indicated for each panel. (C-L) Stage 41 embryos; (A,B) stage 30 embryos. Cyclopamine treatment leads to a strong reduction of Gli1, X-Ptc-1 and X-Ptc-2 expression. Some Gli1 expression remains only in the tailbud (arrowhead in B). Expression of both Gli2 and Gli3 is not affected. Anterior is towards the left. Scale bar: 1.35 mm in A,B; 300 μm in C-L. Image published in: Perron M et al. (2003) Copyright © 2003. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue gli1.S Gli-1, X-gli1 X. laevis Throughout NF stage 28 tail bud brain hindbrain chordoneural hinge ptch1.S patched, patched1, Ptc-1, Ptc1, X-ptc1, XPtc1 X. laevis Throughout NF stage 41 retina brain ganglion eye head region ptch2.L patched 2, patched-2, ptc-2, ptc2, ptch2-a, ptch2-b, xptc 2, xptc-2 X. laevis Throughout NF stage 41 retina brain hindbrain eye stomodeal-hypophyseal primordium pharyngeal arch midbrain heart gli2.S gli4 X. laevis Throughout NF stage 41 retina brain hindbrain forebrain pharyngeal arch otic region midbrain-hindbrain boundary gli3.L XGli-3, Xgli3 X. laevis Throughout NF stage 41 retina brain hindbrain eye forebrain stomodeal-hypophyseal primordium head region otic region gli1.S Gli-1, X-gli1 X. laevis Throughout NF stage 41 brain eye head region pharyngeal arch stomodeal-hypophyseal primordium Image source: Published Experiment + Assay Source Phenotypes and Disease Xla Wt + Cyclopamine + NF39 (in situ hybridization) Fig 7 B Anatomical Phenotype abnormal retinal pigmented epithelium morphology Larger Image Printer Friendly View

Return to previous page