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Fig. 7. Expression patterns of neural crest marker and mesodermal genes in Tbx1 knockdown embryos. (A) Snail2, Sox9, and PCNS expressions in the head of stages 22 and 27 embryos as revealed by whole-mount in situ hybridization. Lateral view, anterior to the left. (B) Distribution of the fluorescent neural crest cells in the cranial neural crest-transplantation experiment. The image is flipped horizontally. (C, D) Myf-5, MyoD, and Tbx6 expressions as revealed by whole-mount in situ hybridization. (C) Stage 37, lateral view, anterior to the left. (D) Stage 16, dorsal view, anterior to top. MO-injected sides (*) are shown by Magenta-GAL staining. Images of MO-injected sides are flipped horizontally to facilitate comparison. Image published in: Tazumi S et al. (2010) Copyright © 2010. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue snai2.L slug, snai2-a, snai2-b, Snail2, xSlu, xslug, XSnail2 X. laevis Throughout NF stage 22 neural crest head cranial neural crest sox9.S cmd1, cmpd1, sox-9, sox9-a, sox9-b, sra1 X. laevis Throughout NF stage 22 head neural crest cranial neural crest myf5.L myf-5, Xmyf-5, Xmyf5 X. laevis Throughout NF stage 37 and 38 head muscle heart tbx6.L XTbx6 X. laevis Throughout NF stage 16 anterior pcdh8l.S pcns X. laevis Sometime during NF stage 22 to NF stage 27 neural crest mesoderm sox9.S cmd1, cmpd1, sox-9, sox9-a, sox9-b, sra1 X. laevis Throughout NF stage 27 head neural crest cranial neural crest otic vesicle optic vesicle epibranchial placode Image source: Published Larger Image Printer Friendly View

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