XB-IMG-48475
Xenbase Image ID: 48475
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Fig. 1. Altered levels of rx1 expression affect blastomere D1.1.1 contributions to retinal cell types. (A–C) Representative cell types from each cell layer were labeled by immunofluorescence for: (A) calbindin-positive cone photoreceptors (red) in the ONL [bar = 40 μm]; (B) GABA-positive (red) horizontal cells in the OINL (note that GABA also stains amacrine cells in the IINL) [bar = 30 μm]; (C) DAPI-positive nuclei (blue) of ganglion cells in the GCL [bar = 30 μm]. For panels A–C, arrowheads note GFP-labeled (green) descendants of blastomere D1.1.1. L, lens. (D–F) Activation of Rx1-GR protein at early (St. 12) and late (St. 16) neural plate stages decreased cell numbers significantly (*p < 0.05 cf. GFP-injected hormone treated controls) for all three cell types. (G–I) Activation of Rx1EnR-GR protein at St. 12 significantly decreased ganglion cells. Activation at St. 16 significantly increased cone and ganglion cell numbers, but horizontal cells were unchanged. (*p < 0.05 cf. GFP-injected hormone treated controls; **p < 0.05 cf. stage 12). Bars indicate SEM. Image published in: Zaghloul NA and Moody SA (2007) Copyright © 2007. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |