XB-IMG-48478
Xenbase Image ID: 48478
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Fig. 4. Changing Rx1 and Pax6 levels/activity at stage 12 alters proliferation and the size of the retina. (A) rx1-GR (rx1) and pax6-GR (pax6) expressing clones contained a significantly larger proportion of mitotic cells than control (β-Gal) clones, whereas loss-of-function of rx1 (rx1EnR) or pax6 (dnpax6) resulted in a significantly decreased mitotic index. The mitotic indices of clones at stage 20 (optic vesicle) that descended from blastomere D1.1.1 were calculated by counting the number of cells in the clone (D–F, blue cytoplasm) that expressed phosphohistone-H3 (D–F, brown nuclei), a marker specific to mitotic prophase. Arrowheads note representative double-labeled cells. Bar = 10 μm. (B) The width of the optic cup at stage 25 was significantly larger when Rx1 or Pax6 levels were increased and significantly smaller when rx1EnR or dnpax6 mRNAs were expressed. (C) Retinal volume at stage 44/45 was significantly larger when Rx1 or Pax6 levels were increased and significantly smaller when rx1EnR or dnpax6 mRNAs were expressed. (*p < 0.05 cf. β-Gal or GFP dex-treated controls). Bars indicate SEM. Image published in: Zaghloul NA and Moody SA (2007) Copyright © 2007. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |