Xenbase Image ID: 48509

FIG. 6. Dorsal Beta-catenin, TGF-Beta, and BMP-antagonist signalling pathways are required for endogenous XHex and cerberus expression. We tested whether the following signalling pathways are required for XHex and cerberus expression: (1) the maternal Wnt/beta-catenin pathway, (2) the dorsalising TGF-Beta signals, and (3) the dorsalising BMP antagonists noggin and chordin. Each of the pathways was blocked by the overexpression of dominant-inhibitory components of the respective pathways. Synthetic mRNA encoding deltaN-XTcf-3, 0.5 ng; XTcf-3, 0.5 ng; dnActRIIB, 1 ng; ActRBII, 1 ng; or BMP4, 1 ng was microinjected subequatorially into the two dorsal blastomeres at the 4-cell stage. At stage 10 0.5 embryos were subjected to whole-mount in situ hybridisation with XHex, cerberus, Xbra, and chordin probes. Representative embryos are shown in each case. The mRNA injected is indicated along the left and the in situ probe is indicated on the top. Inhibition of Beta-catenin or TGF-Beta signalling as well as over-expression of BMP4 blocked endogenous XHex and cerberus expression. Control injections of ActRIIB and XTcf-3 had no effect. Image published in: Zorn AM et al. (1999) Copyright © 1999. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue hhex.L hex, tHex, XHex X. laevis Sometime during NF stage 10 to NF stage 10.5 endomesoderm anterior dorsal marginal zone upper blastopore lip cer1.S cer, cer-1, Cerberus, dand4, LOC108713989, tCerberus, xcer, xcer-1, xcer1 X. laevis Sometime during NF stage 10 to NF stage 10.5 endomesoderm dorsal marginal zone anterior upper blastopore lip tbxt.S bra, brachyury, ntl, t, t-a, t-b, X-bra, Xbra, Xbrachyury X. laevis Sometime during NF stage 10 to NF stage 10.5 mesoderm marginal zone chrd.S chd, chordin, chrd.1, LOC108716939, X-chordin X. laevis Sometime during NF stage 10 to NF stage 10.5 mesoderm involuted dorsal mesoderm upper blastopore lip prechordal plate Image source: Published Larger Image Printer Friendly View

Return to previous page