Xenbase Image ID: 48519

Fig. 2. dkk1 but not frzb induces complete prechordal plates. (A,B) In situ hybridization with sonic hedgehog (Shh) in embryos coinjected at the four-cell stage ventrally with tBR and either dkk1 (A) or frzb (B). Stage-30 embryos are shown from the anterior. Arrowhead indicates the anterior limit of Shh expression. (C-D) in situ hybridization for Shh in embryos injected at the four-cell stage ventrally with nuclear lacZ lineage tracer, tBR and either dkk1 (C) or frzb (D). Stage-16 embryos were cut sagittally; secondary axes are marked by light blue β-gal staining. Arrowheads indicate the anterior limit of secondary Shh expression. Note that the expression in the prechordal plate in (D) is lacking. (E,F) In situ hybridization for goosecoid (gsc) in embryos injected as in A and B. Sagittal sections of stage-14 embryos are shown. Arrowheads indicate the mesodermal zone of gsc expression in the anterior mesoderm of the induced secondary axis (2. Note that mesodermal gsc expression extends anterior to the neuroectodermal in the primary axis (1 and secondary axis induced by dkk1, but is in register with the neural expression in frzb-injected embryos (F). a, anterior; p, posterior. Image published in: Kazanskaya O et al. (2000) Copyright © 2000. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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