Xenbase Image ID: 48564

Fig. 4. p27Xic1 can enlarge the myotome independently of its ability to arrest the cell cycle. One cell of two-cell stage embryos were injected with (A,E) 45 pg p27Xic1, (B,F) 15 pg p27Xic1 NT, (C,G) 50 pg p27Xic1 CT or (D,H) 50 pg p27Xic1 35-96 and β-gal as a tracer (light blue, injected side to left) and allowed to develop until stage 15 (A-D) or 22 (E-H). Dorsal views with anterior downwards (midline indicated by broken white line) demonstrate that phospho- histone-H3 staining (purple) is reduced after injection of all four p27Xic1 constructs (A-D). Transverse sections stained with an antibody against muscle actin (dark blue) indicate that full-length p27Xic1 (A) and p27Xic1 NT (B) can increase the size of the myotome (arrows), while p27Xic1 CT (C) and p27Xic1 35-96 (D) have no effect. Image published in: Vernon AE and Philpott A (2003) Copyright © 2003. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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