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XB-IMG-48755

Xenbase Image ID: 48755


FIG. 3. Milestones of early cardiac development in Xenopus visualized by confocal images of double-immunostained embryos. Embryos selected to represent a chronology of stages were double labeled with anti-tropomyosin (CH1) (red) and anti-fibrillin (JB3) (green) and optically sectioned. For each stage, the set of optical sections (28 to 40 sections, 4 to 6 um thick) was digitally compiled in increments of 4 to 6 sections. Resulting optical thick sections that aid in the visualization of Xenopus heart development are presented. The order of presentation for stages with m ore than one image is ventral to dorsal. Image A is a parasagittal view of a stage 24 hemisectioned embryo. The stage 26 embryo in B is shown from the left lateral view. Images of stages 28 (C and D) and 31 (E) are ventral views of heart regions with the anterior of the em bryos placed at the top. At stage 24, the heart anlage is present but not immunohistochemically active. By stage 26, the first presence of antibody reactivity is noted near the ventral midline (ventral midline lies between arrowheads). At stage 28 (C and D), differentiation of the heart anlage is clear; the pericardial cavity can be discerned. Stage 31 (E)he heart region extends ventrally beyond the pericardial cavity and is slightly bent laterally to the embryo right; the myocardial wall does not yet close. Scale bars as denoted.

Image published in: Kolker SJ et al. (2000)

Copyright © 2000. Image reproduced on Xenbase with permission of the Publisher, Elsevier B. V.

GeneCloneSynonymsSpeciesStage(s)Tissue
tpm1.L alpha-tm, alpha-tropomyosin, CH1X.laevisThroughout NF stage 24somite
paraxial mesoderm
notochord
tpm1.L alpha-tm, alpha-tropomyosin, CH1X.laevisThroughout NF stage 26 to NF stage 28fused heart primordium
myocardium
tpm1.L alpha-tm, alpha-tropomyosin, CH1X.laevisThroughout NF stage 31cardiovascular system
fused heart primordium
heart
muscle
myocardium
fbn2.L JB3X.laevisThroughout NF stage 24notochord
prechordal plate
head mesoderm
presomitic mesoderm
axial mesoderm
fbn2.L JB3X.laevisThroughout NF stage 26 to NF stage 28fused heart primordium
cement gland primordium
muscle
myocardium
fbn2.L JB3X.laevisThroughout NF stage 31cardiovascular system
fused heart primordium
epicardium

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