Xenbase Image ID: 48796

Fig. 2. foxi1-HGR strongly upregulates the expression of ionocyte genes. (A-O) Xenopus embryos were injected at the 2-cell stage with mRNAs encoding foxi1-HGR or foxa1-HGR, activated Notch (ICD) or with both foxi-HGR and ICD, treated with dexamethasone (DEX) at stage 12, fixed at stage 26 and stained for expression of the indicated genes by whole-mount in situ hybridization. foxi1-HGR, but not foxa1-HGR, strongly induced the ectopic expression of ca12 (A) and atp6V0A4 (B), even in the presence of ICD (J,K) but had no effect, or a slight inhibitory effect, on the expression of the ciliated cell marker α-tubulin (C). n-lacZ RNA injections (nβgal) served as a negative control (M-O). Image published in: Quigley IK et al. (2011) Copyright © 2011. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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