Xenbase Image ID: 48800

Fig. 6. ubp1 regulates beta-INC specification. (A,B) Two-color FISH indicates that ubp1 is co-expressed with pendrin, which marks beta-INCs (A), but not with ae1, a marker of alpha-INCs (B). The arrow marks an ae1-expressing cell and the arrowhead marks a ubp1-expressing cell. (C-E) Xenopus embryos were injected with ubp1-HGR RNA at the 2-cell stage, treated with DEX at stage 12 and then fixed at stage 26 to quantify the number of alpha-INCs and beta-INCs using three different approaches: two-label FISH for ae1 (red) and pendrin (green) mRNA (C); pendrin-GFPtr transgenics (green) with ae1 antibody staining (blue) and mRFP (red) (D); or mGFP (green) and B1 antibody staining (red) (E). Arrows mark alpha-INCs. Cells were counted from at least three random fields (196 μm2 of embryo surface) from at least five embryos, typically eight; fields shown are 98 μm2. Error bars indicate ± s.d. All values between a given INC subtype under various conditions are significantly different relative to the control, based on a two-tailed t-test (P≤0.005). Scale bar: 20 μm. Image published in: Quigley IK et al. (2011) Copyright © 2011. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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