Xenbase Image ID: 48828

Fig. 1. Ventral pancreatic bud directs fusion of the dorsal and ventral pancreas. (A) Ventral halves of NF20 Elas-GFP transgenic embryos were recombined with dorsal halves of a wild-type embryo (VtgDwt) and grown to tadpole NF42. Only ventral pancreatic cells will be GFP+. The prospective dorsal and ventral pancreatic buds are illustrated. (B) Fluorescent image of an isolated NF42 whole gut, revealing GFP+ cells in the ventral pancreas, with punctate expression in the dorsal pancreas. (C) Isolated pancreas/liver tissue with no clear demarcation between the dorsal and ventral pancreas. Extensive GFP+ cells are found throughout the dorsal region of the fused pancreas. (D) Annular pancreas (AP) phenotype that developed in a VtgDwt recombinant. GFP+ cells are found in the annular pancreas. (E-G) Color-coded drawings of the images in B-D. Green, GFP+ transgenic pancreatic cells; blue, wild-type pancreatic cells; pink, liver; yellow, intestine. The region of the pancreas that lies behind the stomach is depicted using a lighter shade of green. (H) Schematic illustrating the recombination of dorsal wild-type and ventral transgenic halves (DtgVwt). Only dorsal pancreatic cells will be GFP+. (I) Fluorescent image of an isolated NF42 whole gut showing the fate of GFP+ dorsal pancreas cells. (J) Pancreas/liver tissue was isolated from the whole gut in order to better view the fate of GFP+ cells in the pancreas. There is a sharp demarcation at the dorsal-ventral border; gaps of GFPcells can be seen within the dorsal pancreas. (K) Annular pancreas that developed in a DtgVwt recombination. No GFP+ cells are found in the annular pancreas. (L-N) Color-coded drawings of the images in I-K. Image published in: Jarikji Z et al. (2009) Copyright © 2009. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

Return to previous page