Xenbase Image ID: 48829

Fig. 2. Ventral endoderm cells do not mix with the prepancreatic dorsal endoderm after the archenteron closes. (A) Fluorescent image of a VtgDwt chimeric embryo at NF32 showing GFP expression throughout the entire tadpole, but lacking in the dorsal region. (B) Isolated endoderm from NF35/36 VtgDwt chimeric tadpole showing GFP fluorescence throughout the ventral endoderm, but lacking in the dorsal-most region of the endoderm. (C) Double in situ hybridization for ptf1a (red) and GFP (purple) of endoderm shown in B. No gfp mRNA is detectable in the ptf1a expression domain. (D) Fluorescent image of a DtgVwt chimeric embryo at NF32 showing GFP expression only in the dorsal part of the tadpole. (E) Isolated endoderm from NF35/36 tadpole showing GFP fluorescence only in the dorsal layer of the endoderm. (F) In situ hybridization for ptf1a shown in G. The ptf1a expression domain is located in the dorsal layer of the endoderm. dp, dorsal pancreas. Image published in: Jarikji Z et al. (2009) Copyright © 2009. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue ptf1a.S ptf1a-a, ptf1a-b, Ptf1a/p48, XPtf1a X. laevis Throughout NF stage 32 dorsal pancreatic bud Image source: Published Larger Image Printer Friendly View

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