Xenbase Image ID: 48830

Fig. 3. Microarray analysis of dorsal and ventral pancreatic buds. (A) Schematic illustrating the experimental plan. Individual dorsal and ventral pancreatic buds were isolated from NF38 tadpoles. (B) Diagram of isolated liver and pancreas tissue samples, illustrating the distinct dorsal and ventral regions of the early pancreas, shortly after fusion. Dark blue, dorsal pancreas; light blue, ventral pancreas; pink, liver. (C-F) Whole-mount in situ hybridization on isolated liver/pancreas tissue at NF40 of selected pancreatic genes. dp, dorsal pancreas. (G) RT-PCR analysis of selected pancreatic genes in dorsal and ventral pancreatic bud samples used for the microarray. Image published in: Jarikji Z et al. (2009) Copyright © 2009. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue ins.S ins-a, ins-b, ins1, insulin, insulin I, preproinsulin, xins X. laevis Throughout NF stage 37 and 38 dorsal pancreatic bud neurod1.L neurod, neuroD, neurod1-a, neurod1-b, XNeuroD X. laevis Throughout NF stage 37 and 38 dorsal pancreatic bud pax6.L an2, mgda, pax-6, pax6-a, pax6-b, wagr, XLPAX6, xpax6 X. laevis Throughout NF stage 37 and 38 dorsal pancreatic bud nkx2-2.S nkx2.2, XeNK-2, Xnkx2.2 X. laevis Throughout NF stage 37 and 38 ventral pancreatic bud dorsal pancreatic bud Image source: Published Larger Image Printer Friendly View

Return to previous page