Xenbase Image ID: 48831

Fig. 4. Validation of microarray data. (A,B) Initial confirmation of the data by RT-PCR analysis of selected genes found to be enriched in either the dorsal or ventral bud fraction. (C-E) Dorsal-specific localization of brunol1, insm1 and secretogranin III in isolated liver/pancreas tissue at NF40, except for secretogranin III, which is at NF44. Expression in all three instances is punctate. (F-H) Ventral pancreas-specific expression of tm4sf3, pkc-alpha and agr2 at NF40/42. Expression is localized to a small region in the ventral pancreas, where the two ventral pancreatic buds fuse, and to where the bile duct emerges. Broken lines in C and F indicate the boundary of dorsal and ventral pancreas. Image published in: Jarikji Z et al. (2009) Copyright © 2009. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue celf3.L brunol1, cagh4, celf3-a, celf3-b, erda4, etr-1, etr1, tnrc4 X. laevis Throughout NF stage 40 dorsal pancreatic bud insm1.L IA1 X. laevis Throughout NF stage 40 dorsal pancreatic bud scg3.L secretogranin III, sgiii X. laevis Throughout NF stage 44 dorsal pancreatic bud tspan8.L tm4sf3, transmembrane 4 superfamily member 3 X. laevis Throughout NF stage 40 to NF stage 42 ventral pancreatic bud prkca.L pkc-alpha, pkc-like 2, pkcalpha, XPKCI X. laevis Sometime during NF stage 40 to NF stage 42 ventral pancreatic bud agr2.L gob-4, np77, pdia17, xagr2 X. laevis Sometime during NF stage 40 to NF stage 42 ventral pancreatic bud Image source: Published Larger Image Printer Friendly View

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