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Fig. 3. WMISH analysis. (A–U) Results of in situ hybridization at three stages are shown for seven genes showing patterns of interest. These genes show expression in one or more of the following: GRP (blue arrows); heart or branchial arches (red arrows); kidney (green arrows). Stage 15 to 19 embryos are viewed dorso-posteriorly with anterior to the top to examine GRP expression (shown schematically in V). Stage 26 to 29 and stage 33 to 36 embryos are viewed laterally with anterior to the left (shown schematically in W and X, respectively). Image published in: Fakhro KA et al. (2011) Copyright © 2011. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue tgfbr2 aat3, faa3, lds1b, lds2b, mfs2, riic, taad2, tgfbeta-rii, tgfr-2 X. tropicalis Throughout NF stage 18 neural plate anterior neural fold tgfbr2 aat3, faa3, lds1b, lds2b, mfs2, riic, taad2, tgfbeta-rii, tgfr-2 X. tropicalis Sometime during NF stage 26 to NF stage 29 and 30 heart heart primordium cardiovascular system branchial arch pharyngeal arch mandibular arch hyoid arch notochord somite olfactory region head hypaxial muscle paraxial mesoderm tgfbr2 aat3, faa3, lds1b, lds2b, mfs2, riic, taad2, tgfbeta-rii, tgfr-2 X. tropicalis Sometime during NF stage 33 and 34 to NF stage 35 and 36 heart pronephric kidney heart primordium cardiovascular system branchial arch pharyngeal arch mandibular arch hyoid arch cranial ganglion somite paraxial mesoderm eye lens pronephric duct notochord rock2 xROK X. tropicalis Throughout NF stage 18 neural groove anterior neural fold neural plate chordal neural plate rock2 xROK X. tropicalis Sometime during NF stage 26 to NF stage 29 and 30 heart heart primordium cardiovascular system branchial arch pharyngeal arch mandibular arch hyoid arch spinal cord cranial ganglion dorsal tail fin trigeminal ganglion rock2 xROK X. tropicalis Sometime during NF stage 33 and 34 to NF stage 35 and 36 heart heart primordium cardiovascular system branchial arch pharyngeal arch mandibular arch hyoid arch otic vesicle cranial ganglion trigeminal ganglion spinal cord notochord dorsal tail fin eye olfactory region myocardium neural crest galnt11 galnac-t11, pp-gantase 11 X. tropicalis Throughout NF stage 18 roof plate archenteron anterior posterior anterior neural fold neural plate galnt11 galnac-t11, pp-gantase 11 X. tropicalis Throughout NF stage 26 to NF stage 35 and 36 pronephric kidney branchial arch pharyngeal arch mandibular arch hyoid arch eye spinal cord cranial ganglion lateral line placode olfactory placode cement gland lrrc8a agm5, lrrc8, swell1 X. tropicalis Throughout NF stage 18 roof plate archenteron neural plate chordal neural plate anterior neural fold neural groove neuroectoderm neural crest lrrc8a agm5, lrrc8, swell1 X. tropicalis Sometime during NF stage 33 and 34 to NF stage 35 and 36 heart pronephric kidney heart primordium cardiovascular system branchial arch pharyngeal arch mandibular arch hyoid arch ventral ventral blood island brain hindbrain spinal cord eye midbrain otic vesicle olfactory region chordoneural hinge stomodeal-hypophyseal primordium nek2 nek2a X. tropicalis Throughout NF stage 18 roof plate archenteron epidermis epidermis outer layer nek2 nek2a X. tropicalis Sometime during NF stage 26 to NF stage 29 and 30 pronephric kidney branchial arch pharyngeal arch mandibular arch hyoid arch pronephric duct neural tube posterior neural tube anterior neural tube spinal cord eye dorsal tail fin cloaca otic vesicle brain hindbrain tail bud chordoneural hinge nek2 nek2a X. tropicalis Sometime during NF stage 33 and 34 to NF stage 35 and 36 heart pronephric kidney branchial arch pharyngeal arch mandibular arch hyoid arch tail bud chordoneural hinge hindbrain brain midbrain-hindbrain boundary otic vesicle spinal cord olfactory region stomodeal-hypophyseal primordium eye nup188 X. tropicalis Sometime during NF stage 26 to NF stage 35 and 36 pronephric kidney branchial arch pharyngeal arch mandibular arch hyoid arch eye brain hindbrain spinal cord somite paraxial mesoderm tail bud chordoneural hinge ventral ventral blood island pronephric duct cranial ganglion neuroectoderm neural crest lateral line placode otic vesicle olfactory region stomodeal-hypophyseal primordium greb1 X. tropicalis Sometime during NF stage 26 to NF stage 29 and 30 pronephric kidney branchial arch pharyngeal arch mandibular arch hyoid arch eye otic vesicle spinal cord chordoneural hinge tail bud brain hindbrain midbrain midbrain-hindbrain boundary neural tube anterior neural tube posterior neural tube lateral line placode somite posterior cardinal vein greb1 X. tropicalis Throughout NF stage 19 anterior neural fold ectoderm epidermis neuroectoderm neural crest neural plate nup188 X. tropicalis Throughout NF stage 19 neural plate chordal neural plate anterior neural fold epidermis outer layer ectoderm neuroectoderm epidermis lrrc8a agm5, lrrc8, swell1 X. tropicalis Sometime during NF stage 26 to NF stage 29 and 30 branchial arch pharyngeal arch mandibular arch hyoid arch eye brain hindbrain midbrain-hindbrain boundary spinal cord lateral line placode olfactory placode otic placode ventral ventral blood island greb1 X. tropicalis Sometime during NF stage 33 and 34 to NF stage 35 and 36 heart heart primordium cardiovascular system branchial arch pharyngeal arch mandibular arch hyoid arch tail bud chordoneural hinge spinal cord eye brain hindbrain midbrain-hindbrain boundary olfactory region lateral line placode head Image source: Published Larger Image Printer Friendly View

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