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Xenbase Image ID: 48868

Fig. 1. A −6Kb:hhex:gfp transgene recapitulates hhex expression. (A) Diagram of the Xenopus laevis hhex genomic locus and −6.0Kb:hhex:gfp transgene. (B) The transgene recapitulates hhex expression in the anterior endoderm from blastula (st9.5) to stages 25. Transgenic embryos assayed by in situ hybridization for hhex or gfp mRNA and for GFP fluorescence with a confocal (st9.5 and 10) or stereomicroscope (st18–35). Embryos from st9.5 to 18 are bisected; in st9.5–11, dorsal is left; and in st18–35, anterior is right. The GFP image at st35 shows both a transgenic (top) and non-transgenic embryos (bottom). Two independent transgenic lines exhibited identical expression patterns. Arrowheads indicate endothelial cells (black), thyroid (white), and liver bud (yellow).

Image published in: Rankin SA et al. (2011)

Copyright © 2011. Image reproduced with permission of the Publisher, Elsevier B. V.

hhex.LXHex, tHex, hexX.laevisThroughout NF stage 9endoderm
hhex.LXHex, tHex, hexX.laevisThroughout NF stage 10endoderm
hhex.LXHex, tHex, hexX.laevisThroughout NF stage 18endoderm
liver primordium
liver diverticulum
hhex.LXHex, tHex, hexX.laevisThroughout NF stage 25endoderm
liver primordium
endothelial cell
vascular endothelium
alimentary system
hhex.LXHex, tHex, hexX.laevisThroughout NF stage 35 and 36foregut
liver primordium
thyroid primordium
posterior cardinal vein
alimentary system

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