Xenbase Image ID: 48870

Fig. 3. Mapping Nodal and Wnt-responsive cis-elements. (A) Representative examples of GFP fluorescence from hhex:gfp deletion constructs in bisected transgenic gastrulae. Arrowheads show ectopic GFP. (B) Relative luciferase activity of early gastrulae injected with hhex:luc deletion constructs into either dorsal C1 (green), the ventral C4 cells (red) or (C) into the A4 ectoderm cells with xnr1 RNA (50 pg; gray) or stabilized pt-β-catenin RNA (250 pg; black). (B–C) Histograms show the average normalized luciferase activity and standard deviation from injections performed in biological triplicate. Representative examples from 3 independent experiments are shown. *p < 0.05 in Student T-tests when compared to injection of the same reporter alone; ns = no statistical difference. The NRE localized between −0.44 and −0.38 Kb, and the WRE between −0.65 and −0.55 Kb. Image published in: Rankin SA et al. (2011) Copyright © 2011. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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