Xenbase Image ID: 48981

Fig. 2. AP2a induces NB genes in neuralized ectoderm explants and is essential for NC induction by FGF and Wnt. (A) Although explants neuralized by Noggin express zic1, AP2 activates coexpression of pax3 and msx1. RT-PCR for NB markers pax3, msx1, and zic1 after injection of Noggin (lane 4), ap2a (lane 5), or ap2a combined with Noggin (lane 6). Controls are shown in lanes 1–3 (SI Materials and Methods). (B) FGF controls ap2a at the NB in a β-catenin–dependent manner. RT-PCR analysis of ap2a in neuralized explants (lanes 4–7) and treated with bFGF (lane 5), CHX (lane 6), or CHX followed by bFGF (lane 7). Controls are shown in lanes 1–3 (SI Materials and Methods). (C) Expansion of ap2a by FGF is abolished by β-catenin depletion. Injections of a constitutively active FGFR4 (caFGFR4), β-catenin MO, or both at stage 17, anterior views. (D) AP2a restores snail2 expression in both FGF and β-catenin morphants. Injections of ap2a, FGF8MO, and βcatMO alone and combined. (E) AP2a mediates NB induction by FGF and Wnt. AP2a morphants lack snail2, hairy2, and msx1 expression even with FGF or Wnt GOF. Injections of caFGFR4, AP2aMO, and activated β-catenin (δβcat) alone or combined at stage 17, anterior views. The star indicates the injected side. Image published in: de Crozé N et al. (2011) Copyright © 2011. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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