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Figure 4. Sdf1 Stabilizes Cell Polarity Induced by Cell Interactions(A–D) Two-plane confocal image to show cell protrusions (red) and cell shape (green) in single cells (A and B) and groups (C and D), with (+) or without (−) Sdf, as indicated.(E–H) Orientation of cell protrusions analyzed from time-lapse movies in single cells (E and F) and groups (G and H), with (F and H) or without (E and G) Sdf1.(I–K) Size (I), duration (J), and numbers (K) of protrusions are shown for each condition (n = 50 per condition). Gray bar, single cells; black bar, group of cells. ∗p < 0.05; ∗∗∗p < 0.005.(L–U) FRET analysis of Rac1 activity in single, outer, and inner cells without (L–N) and with (O–Q) Sdf1 shows that Rac1 activity distribution is depending on cell contacts.(R) Levels of Rac1 activity in outer cells at the front (n = 26) or at the back (n = 25) of an explant with (+) or without (−, n = 20) Sdf1 and inner cells with (+, n = 6) or without (−, n = 6) Sdf1. ∗∗∗p < 0.005.(S–U) Summary of Rac1 activity distribution in single (S), outer (T), and inner (U) cells exposed to Sdf1. Circles under each bar represent different types of Rac1 polarities, which were quantified. Error bars show standard deviation. See also Figure S3. Image published in: Theveneau E et al. (2010) © 2010 ELL & Excerpta Medica. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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