Xenbase Image ID: 49016

Figure 5. N-Cadherin-Dependent Contacts Are Required for Collective Chemotaxis(A–F) N-cadherin expression in premigratory (A–C) and migratory (D–F) NC cells analyzed by whole mount in situ hybridization (A and D) and immunostaining (B, C, E, and F); NC cells streams are delimited by dotted lines.(G and H) N-cadherin loss-of-function using an antisense Morpholino (n = 87).(I and J) Full-length N-cadherin overexpression (n = 40).(K–S) NC cells labeled with rhodamine-dextran (RD) were grafted into unlabeled embryos and NC migration was monitored looking at the RD fluorescence. Immunostaining on sections for N-cadherin (N and O), β-catenin (P and Q), and p120-catenin (R and S) are shown in low and high magnification. Blue, DAPI staining. Scale bar, 20 μm.(T–V) Tracks of control cells ([J], n = 16) and NC pretreated with a control IgG ([K], n = 22) or with N-cadherin blocking antibody NCD2 ([L], n = 27) and exposed to Sdf1 showing that N-cadherin inhibition strongly blocks chemoattraction toward Sdf1. Chemotaxis index for each condition is shown in (W). b, branchial; h, hyoid. Error bars show standard deviation. See also Movies S7 and S8. Image published in: Theveneau E et al. (2010) © 2010 ELL & Excerpta Medica. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Gene Synonyms Species Stage(s) Tissue cdh2.L cadherin-2, cdh2-a, cdh2-b, N-Cad, N-cadherin, ncad X. laevis Throughout NF stage 19 to NF stage 26 cranial neural crest mandibular crest hyoid crest branchial crest anterior branchial crest posterior branchial crest neural tube posterior neural tube anterior neural tube eye primordium optic field optic vesicle twist1.S twist, twist1-a, twist1-b, X-twi, Xtwi, Xtwist X. laevis Throughout NF stage 26 cranial neural crest mandibular crest hyoid crest branchial crest anterior branchial crest posterior branchial crest cdh2.L cadherin-2, cdh2-a, cdh2-b, N-Cad, N-cadherin, ncad X. laevis Throughout NF stage 26 migratory neural crest cell ctnnb1.S B-catenin, beta catenin, beta-catenin, ctnnb1-a, ctnnb1-b, β-catenin X. laevis Throughout NF stage 26 migratory neural crest cell ctnnd1.L p120, p120(ctn), p120-catenin, p120-ctn, p120ctn, Xp120, Xp120(ctn) X. laevis Throughout NF stage 26 migratory neural crest cell Image source: Published Larger Image Printer Friendly View

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