Xenbase Image ID: 49017

Figure 6. N-Cadherin-Dependent Cell Interactions Prevent Formation of Cell Protrusions through Local Inhibition of Rac1 at Cell Contacts(A–D) Embryos were injected with mbRFP (blue) at the 2 cell stage and with N-cadherin MO/mbGFP at the 32 cell stage to generate a mosaic expression of the MO. Two-plane confocal image to show cell protrusions (red) and cell shape (green) in control cells (A and B) and N-cadherin MO cells (C and D), with (+) or without (−) Sdf1, as indicated. N-cadherin loss-of-function induces formation of ectopic cell protrusions overlapping with neighboring cells ([C and D], arrowheads) regardless of Sdf1.(E) Size of inner cell protrusions (n = 10).(F–G′) In vivo, confocal images of migrating NC cells labeled with mbGFP and nRFP grafted into a control embryo. Not all the cells are labeled. Region shown equivalent to Figure 2O (middle of NC stream). Control cells (F and F′) have clear cell-cell boundaries while N-cadherin-Mo-injected cells (G and G′) have high membrane activity and show overlapping protrusions. Labeled cells surrounded by nonlabeled cells are presented in high magnification in F′ and G′. Scale bars, 15 μm.(H–J) FRET analysis of Rac1 activity distribution in control outer cells or outer cells treated with NCD2 antibody as indicated (n = 27). Scale bars, 10 μm.(K) Rac1 activity at the cell contacts region (n = 18).(L) Global Rac1 activity in outer cells (n = 29) ∗p < 0.05, ∗∗∗p < 0.005. Error bars show standard deviation. See also Figure S4 and Movie S9. Image published in: Theveneau E et al. (2010) © 2010 ELL & Excerpta Medica. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

Return to previous page