Xenbase Image ID: 49181

Figure 5. Wnt-Dependent TCF3 Phosphorylation Relieves Transcriptional Repression(A and B) Xenopus TCF3 phosphorylation mutants enhance the dorsoanterior fate (A) and inhibit Wnt8-dependent reporter activation of BREm-Luc (B). TCF3 RNAs were injected into four animal pole blastomeres at the eight-cell stage. Representative phenotype classes based on cement gland size at stage 28 are shown. Class I, more than double the size of a normal cement gland; class II, more than triple the size of a normal cement gland.(C) Mouse TCF3 P2/3/4 mutant inhibits Wnt-dependent reporter activation in HEK293 cells more efficiently than wild-type TCF3 (p < 0.05 for 1 ng and p < 0.01 for 10 ng, respectively). TOP/FOP is the ratio of STF reporter activity over STM reporter activity, presented as means ± SD. Image published in: Hikasa H et al. (2010) Copyright © 2010. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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