Xenbase Image ID: 49247

Fig. 5. Dkk1GFP represses fgf-8 expression in the regenerating blastema, but not expression of fgf-10, Lmx-1, Hoxa-13 or msx-2. (A) Experimental scheme. One heat-shock was applied to tadpoles at 5 dpa (blue line). Wild-type and hsDkk1GFP tadpoles were fixed 8 h after the heat-shock. (B) In situ hybridization on sectioned samples of blastemas. Sectioned samples were hybridized with fgf-8 (a and b), fgf-10 (c and d), Lmx-1 (e and f), Hoxa-13 (g and h) or msx-2 (i and j). To guarantee the correct comparisons of the gene expression level, wild-type (a, c, e, g and i) and hsDkk1GFP (b, d, f, h and j) tadpole sections treated exactly the same way. Arrowheads show amputation level (knee level). D, dorsal; V, ventral. Scale bar: 100 μm. Image published in: Yokoyama H et al. (2007) Copyright © 2007. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue fgf8.L AIGF, FGF-8, fgf-8b, fgf8a, fgf8b, MGC79739, Xfgf-8, Xfgf8 X. laevis Throughout NF stage 54 epithelium limb bud blastema hindlimb bud distal fgf10.S fgf-10 X. laevis Throughout NF stage 54 limb bud blastema distal mesenchyme hindlimb bud msx2.L Hhox 7.1 X. laevis Throughout NF stage 54 limb bud blastema dorsal mesenchyme hindlimb bud hoxa13.S hoxa13-A, Xhoxa13 X. laevis Throughout NF stage 54 limb bud blastema hindlimb bud mesenchyme distal mesenchyme Image source: Published Larger Image Printer Friendly View

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