Xenbase Image ID: 49378

Figure 2. The phenotype of FoxC1 depleted gastulae. A:Xenopus FoxC1 pseudoalleles aligned against the reverse complement of the morpholino oligo used in the study using Clustal alignment in Macvector. B: FoxC1 MO (MO) blocks translation of FoxC1 mRNA (FoxC1) in a dose-responsive fashion, but does not block translation of a protected FoxC1 RNA, (MOR-), in an in vitro translation assay. C: HA-tagged Fox1 mRNA was injected alone or together with different doses of FoxC1 MO at the 2-cell stage to confirm a reduction of FoxC1 translation. D–G: Wild type sibling embryos and embryos injected with 40 ng MO (MO), or 40 ng MO and 50 pg MO-R FoxC1 mRNA (MO+RNA) were cultured until early (stage 10), mid (stage 11), and late gastrula (stage 11.5) stages before freezing and analyzing by real time RT-PCR for the expression of Mespo (D), Endodermin (E), Bix4 (F), and CK1ϵ (G). Expression levels are normalized to ODC. H,I: Whole mount in situ hybridization on FoxC1-depleted mid-gastrulae (bottom) as compared to wild type sibling embryos (top) showing increased expression of CK1ϵ (H) and Bix4 (I) after FoxC1 depletion. Image published in: Cha JY et al. (2007) Copyright © 2007. Image reproduced with permission of the Publisher, John Wiley & Sons. Gene Synonyms Species Stage(s) Tissue foxc1.S fkhl7, foxc1-a, foxc1-b, freac-3, freac3, igda, ihg1, irid1, rieg3, xfd-11 X. laevis Throughout NF stage 11 mesoderm marginal zone Image source: Published Larger Image Printer Friendly View

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