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Fig. 6. FoxO4MO injection affects eye, heart and cranial crest development. (A) FoxO4 depletion results in lack of eye structures. Head of an unilaterally FoxO4MO injected embryo (40 ng/blastomere) at stage 39 (lateral view). The eye is almost absent. Middle: Pax-6 whole mount in situ hybridization of control and unilaterally FoxO4MO injected embryos at stage 21. Bottom: Otx-2 whole mount in situ hybridization of control and unilaterally FoxO4MO injected embryos at stage 25. (B) FoxO4 depletion affects cranial crest and jaw development. In situ hybridization of unilaterally FoxO4MO injected embryos with the neural crest markers FoxD3 at stage 23 and AP2α at stage 33. Red arrows indicate the lack of FoxD3 and AP2α expression. Bottom: Alcian blue stained control and unilaterally FoxO4MO injected embryo (30 ng/blastomere) at stage 47. (C) FoxO4 depletion results in failure of heart development. Top: lateral view on control and bilaterally FoxO4MO injected embryo at stage 41. Right: control heart of stage 43 embryo and heart of FoxO4MO injected embryo (20 ng/blastomere at 2-cell stage). Bottom: control- and unilaterally FoxO4Mo injected embryos. myocd and MHCα in situ hybridization reveals reduced expression at the injected side. In situ hybridization of unilaterally FoxO4MO injected embryos with Nkx2.5, GATA-5 and Tbx20. Red asterisks indicate the side of injection. ba, branchial arches; c, conus; ch, ceratohyale; mc, Meckel's cartilage; v, ventricle. Percentages of phenotypes are given in supplementary Table S7. Image published in: Schuff M et al. (2010) Copyright © 2010. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue pax6.L an2, mgda, pax-6, pax6-a, pax6-b, wagr, XLPAX6, xpax6 X. laevis Throughout NF stage 21 optic field neural tube otx2.S otx-2, otx2-a, otx2-b, otxA, Xotx-2, Xotx2 X. laevis Throughout NF stage 25 brain forebrain optic field olfactory placode foxd3.L fkh6, foxd3-a, foxd3-b, XFD-6, xfoxd3 X. laevis Throughout NF stage 23 neural crest cranial neural crest myocd.L myocardin, SRF cofactor protein X. laevis Throughout NF stage 41 heart cardiac mesoderm ap2a2.L alpha-adaptin, LOC108714918 X. laevis Throughout NF stage 33 and 34 head region neural crest anterior branchial crest posterior branchial crest myh6.S aMHC, ck231, MHC, MHC-alpha, MHCa, MHCalpha, muz, muzak, XCMHC X. laevis Throughout NF stage 41 heart cardiac mesoderm nkx2-5.L AR2, csx, Nkx-2.5, nkx-2.5, nkx2-5-a, nkx2-5-b, nkx2.5, tinman, XNkx-2.5, XNkx2-5 X. laevis Throughout NF stage 41 heart cardiac mesoderm gata5.S gata-4a, GATA-5, gata5-a, gata5-b, xGATA-5, xGATA-5a, xGATA-5b, xGATA5, xGATA5a, xGATA5b X. laevis Throughout NF stage 41 heart cardiac mesoderm tbx20.L XTBX20 X. laevis Throughout NF stage 41 heart cardiac mesoderm Image source: Published Larger Image Printer Friendly View

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